Purity ≥95% by SDS PAGE. Recombinant Luciferase is a luciferase expressed from a cloned gene from the North American firefly (Photinus pyralis) that provides the reliability and dependability needed for performing research or producing kits using bioluminescence reagents to detect ATP or luciferin substrates. A recombinant source eliminates the possibility of seasonal and regional variability that may be found in luciferase purified from natural sources. Luciferase catalyzes the oxidation of reduced flavin mononucleotide (FMNH2) and a long chain aldehyde by molecular oxygen to yield FMN, the corresponding acid, H2O and light. EC No:: 1.14.14.3 CAS No: 61970-00-1 Molecular Weight: 61kD Source: Recombinant (E. coli, Photinus pyralis) Purity: ≥95% by SDS PAGE Concentration: 10-15mg/ml (A278,E=75(mg/ml)-1cm-1)