Purity ~95% (SDS-PAGE). LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. Source: Recombinant protein corresponding to aa1-311 from renilla reniformis LUC, fused to His-Tag at N-terminal, expressed in E. coli. Molecular Weight: ~38.5kD (335aa), confirmed by MALDI-TOF Biological Activity: Specific activity is >1X10e9 light units/mg. One luciferase enzyme units will produce one Relative Light Unit (RLU) at pH 7.5 at 25°C. AA