Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Description
Purity ≥ 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. A glycoprotein of MW 20kD containing internal disulfide bonds. It induces the survival, proliferation, and differentiation of neutrophilic granulocyte precursor cells and functionally activates mature blood neutrophils. Among the family of colony-stimulating factors, G-CSF is the most potent inducer of terminal differentiation to granulocytes and macrophages of leukemic myeloid cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, Interleukin-1 and GM-CSF. Prostaglandin E2 inhibits the synthesis of G-CSF. In epithelial, endothelial, and fibroblastic cells secretion of G-CSF is induced by Interleukin-17. Recombinant Human G-CSF is a single, non-glycosylated, polypeptide chain containing 175 amino acids and having a molecular mass of 18.8kD Source: E. coli Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be M