Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Description
Purity ≥95% by SDS PAGE. Human granulocyte colony-stimulating factor(hG-CSF) is one of the hematopoietic growth factors which plays an important role in stimulating proliferation, differentiation, and functional activation of blood cells. In addition to its effect on hematopoiesis, G-CSF also enhances neutrophil functions against bacteria, fungi, and tumor cells mediated by antibody-dependent cell-mediated cytotoxicity. Recombinant hG-CSF was expressed in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Molecular Weight: 18.8kD (175amino acids including N-terminal methionine) Sequence: MTPLGPASSL/ PQSFLLKCLE/ QVRKIQGDGA/ ALQEKLCATY/ KLCHPEELVL/ LGHSLGIPWA/ PLSSCPSQAL/ QLAGCLSQLH/ SGLFLYQGLL/ QALEGISPEL/ GPTLDTLQLD/ VADFATTIWQ/ QMEELGMAPA/ LQPTQGAMPA/ FASAFQRRAG/ GVLVASHLQS/ FLEVSYRVLR/ HLAQP Biological Activity: Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphobla