Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Description
Purity ~98% (SDS-PAGE, HPLC). G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Source: Recombinant protein corresponding to human Granulocyte Colony Stimulating Factor, a single non-glycosylated polypeptide chain containing 174aa, expressed in E. coli. Molecular Weight: ~18.7kD (174aa) Applications: Suitable for use in SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Biological Activity Fully biologically active when compared to standard. The ED50 as calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is <0.1ng