Granulocyte Colony Stimulating Factor, Recombinant, Human (G-CSF)
Description
Purity ≥98% by SDS-PAGE. Endotoxin: ≤0.1 ng/ug. G-CSF is a hematopoietic growth factor that stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation. Human and murine G-CSF are cross-species reactive. Recombinant human G-CSF is an 18.7 kDa protein consisting of 174 amino acid residues. Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is ≤ 0.1ng/ml, corresponding to a specific activity of ≥ 1x10e7 units/mg. AA Sequence: TPLGPASSLP QSFLLKCLEQ VRKIQGDGAA LQEKLCATYK LCHPEELVLL GHSLGIPWAP LSSCPSQALQ LAGCLSQLHS GLFLYQGLLQ ALEGISPELG PTLDTLQLDV ADFATTIWQQ MEELGMAPAL QPTQGAMPAF ASAFQRRAGG VLVASH