The C2C12 cell line is an immortal line of mouse skeletal myoblasts, originally established from satellite cells of the thigh muscle of a two month old C3H mouse 70 hours after a crush injury (Yaffe et al., 1977). The cells differentiate rapidly and produce extensive contracting myotubes expressing characteristic muscle proteins. The subclone C2C12 was isolated and selected for its ability to differentiate rapidly and produce extensive contracting myotubes expressing characteristic muscle proteins by HM Blau in 1985.