This assay employs the competitive enzyme immunoassay technique. An antibody specific for Estradiol has been pre-coated onto a microtiter plate. Endogenous Estradiol in samples or standards competes with an Estradiol-horseradish peroxidase conjugate for binding to the coated antibody. After incubation the unbound conjugate is washed off. The amount of bound peroxidase conjugate is inversely proportional to the concentration of Estradiol in the sample. After addition of the substrate solution, the intensity of color developed is inversely proportional to the concentration of Estradiol in the sample. Estradiol concentration in the sample is calculated through a calibration curve. Sample Types: rat serum. Range: 5 - 1280 pg/ml. Sensitivity: 2.5 pg/ml. Time: 4 hours ? Estradiol (E2), also spelled oestradiol, is a steroid, an estrogen, and the primary female sex hormone. It is named for and is important in the regulation of the estrous and menstrual female reproductive cycles. Estradiol is essential for the development and maintenance of female reproductive tissues such as the breasts, uterus, and vagina during puberty, adulthood, and pregnancy, but it also has important effects in many other tissues, including bone, fat, skin, liver, and the brain. While estrogen levels in men are lower compared to those in women, estrogens have essential functions in men, as well. It is found in most vertebrates and crustaceans, insects, fish, and other animal species. Estradiol is produced especially within the follicles of the female ovaries, but also in other endocrine (i.e., hormone-producing) and nonendocrine tissues (e.g., including fat, liver, adrenal, breast, and neural tissues). Estradiol is biosynthesized from cholesterol through a series of chemical intermediates. One principal pathway involves the generation of androstenedione, which is converted into estrone by aromatase and then by 17β-hydroxysteroid dehydrogenase into estradiol. Alternatively, androstenedione can be converted into testosterone, an androgen and the primary male sex hormone, which in turn can be aromatized into estradiol. In female rodents, the determination of estradiol is a useful marker in evaluating and monitoring the state of the reproductive functions and pregnancy as well.
Size
1 x 96 well
Concentration
n/a
Applications
RUO
Other Names
Estradiol, 17 b estradiol, 17 beta estradiol. Estrogen