LACTATE is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. This lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lambda ex/em = 530/585 nm, is proportional to the lactate concentration in the sample. Sample types: cell culture media, plasma, serum
Size
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Concentration
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Applications
For quantitative determination of L-lactate (L-lactic acid) and evaluation of drug effects on its metabolism.