Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to llama IgG2 or IgG3 are observed.
Size
0.5 mg
Concentration
1.02 mg/mL
Applications
ELISA, Flow Cytometry, Western Blot. Llama IgG1 isotype control can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.Llama IgG1 is buffered in 0.075 M Tris, 0.375 M NaCl, 25% glycerol.