Tumorigenecity: Yes, produces tumors in nude mice consistent with type II bronchiolar adenocarcinoma; Isoenzyme: G6PD, B;PGM1,1;PGM3,2;ES-D,1;Me-2,2;AK-1,1;GLO-1,2 Histopathology: carcinoma, squamous cell; grade IV; Subculture:Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or 37C) until the cells detach (about 10min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Split ratio:1:2; Media change:2 to 3 times per week; Reverse transcritase:Production.