Human osteoblasts are a highly specialized cell type of mesenchymal origin involved in bone formation and remodeling. In vivo, they produce the osteoid, a extracellular matrix rich in collagen type I. In the course of their natural maturation process into osteocytes, called osteogenesis, they become embedded in the bone matrix and stop proliferating. AcceGen Biotech Human Osteoblasts (HOB) are produced at AcceGen Biotech's cell culture facility and are available from knee and hip femoral tissue. Shortly after isolation, all AcceGen Biotech Human Osteoblasts are cryopreserved at passage 2 (P2) using AcceGen Biotech's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at AcceGen Biotech.