1-429. MW: 48.45kD. Purity >90% as determined by SDS-PAGE. This methylase recognizes the double-stranded sequence GTGCAC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the ApaLI endonuclease.
Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI, and SapI restriction-modification systems in Escherichia coli. Xu S.-Y., Xiao J.-P., Ettwiller L., Holden M., Aliotta J., Poh C.L., Dalton M., Robinson D.P., Petronzio T.R., Moran L., Ganatra M., Ware J., Slatko B., Benner J. II Mol. Gen. Genet. 260:226-231(1998)