The liver, which is mainly formed by highly specialized cells called hepatocytes, is the main metabolic organ of the body performing glycolysis, glycogenesis, amino acid metabolism, lipid metabolism, and ureagenesis. In addition,hepatocytes play a key role in elimination of toxic substances. Therefore, cultured human hepatocytes are perfectly suited for in vitro metabolism and detoxification studies. AcceGen Biotech Human Hepatocytes (HHpC) are produced at AcceGen Biotech's cell culture facility from the normal human liver tissue of single Donors.Immediately after isolation, all AcceGen Biotech Human Hepatocytes are cryopreserved as freshly prepared cells using AcceGen Biotech's proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains 3-6 million viable cells with a guaranteed adherence rate of > 50% after thawing.Note: Under standard in vitro cell culture conditions, mature human hepatocytes usually do not survive for longer than 10-14 days and do not proliferate.