??????????????????????豸?????????????????????????????豸??
????????????????飺?????? iPSC ??????????????
???? ?????????????? ??????????? ???????????У??????????????????????????????????????????????????????????? ?????????????? ??????????????????????????????????????????????????????????????????????????????????????????????????????? ???? ??????????????? [1]??????????????ж????????????
REFERENCES[1] hPSC-CM non patterned (left image) and on line micropatterns (right image) imaged by fluorescence microscopy. Dussaud S., Jouve C., Hulot J.S., 2018. These images were obtained through the courtesy of Professor Hulot (H?pital Europ??en Georges-Pompidou) and his team, using 4Dcell products. [2] Max R. Salick, Wendy C. Crone. et al. Micropattern Width Dependent Sarcomere Development in Human ESC-Derived Cardiomyocytes, Biomaterials. 2014, May. 35(15): 4454-4464.
????3D ????????????????? iPSC-CM ????????
???? ?????????????????? ??????????? ???????????У????????????????????????????????????????????????????????????? 3D ??????????? ???? 3D ?????????????hiPSC-CM ??????????????????????????????? iPSC-CM ????Ч????? ???? ????????????????????????????????????????????A????????????????????????????????????????????????????????????????????? (B)(C) ????????????
REFERENCESFu, F. et al., (2018). Bioinspired living structural color hydrogels. Science Robotics, 3(16), eaar8580. Ribeiro AJ, et al. Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness, Proc Natl Acad Sci U S A. 2015;112(41):12705-10
?????????????????????????????????
???? ??????????????????????????л????????????? ??????????? ????????????????????????????????????????????????????е???????λ?????????????????????????????????????????????????????????????????????????????????????????????????? ????????? ??? 4Dcell ???????????? PDMS ?????????????????????????λ?????????????????? ?????????X??????????????????????? 1 nN ??????????????????????????????? ????(A) ????????????????? (B) ?????????????????????????????? ?FSA ?????????
REFERENCES[1] Trichet, L. et al. (2012). Proceedings of the National Academy of Sciences, 109(18), 6933-6938. [2] Gupta, M. et al. (2015). Methods in cell biology, 125, 89-308.
?????????????????????????????????????????????
????????????????????????????????????? ??????????? ?? hESC-??????????????????????????? 2D ????????У??????????????????????????????????????????????????????????? ????????????????????? ?????У??????????????????????????????????????????????????????????????????????ê????????????????????????????? CM ?????????-???????Ч??? ???? hESC ???????и?????????????????????????????????????????????????????????????????[1]??
?塢Durotaxis ?????????????????????
Durotaxis ??????????????????? ????
Durotaxis??haptotaxis??????????? ????????????????????????????????????????????á???????ê??????????Χ??????????????????????????????????????????????к????????????? Durotaxis ?? Haptotaxis ???????? ????
REFERENCE[1] R??he, J. et al. (2016). Langmuir, 32(46), 12190?C12201. ???????????????????????????????? ????????????????????? ??????????????????????????????????????????????κ??????????????????????? ???????????????????????????????????????????????????????????????????????????????????????????????? ???????? 1 kPa ?????????? HeLa ????????????? [1]?? ?????η????????飺????鵼?????????
???? ??????????????????????????????? ??????????? ???????????У??????????????????????????? ??????????????????????????????????????????????????????????????????????????й?? ??μ????????? ??Χ????η???????????????????????????????????????????????????????????? ????????????????????????????????γ???? [1]?????????? [2] ??????????????????ü???????鵼???????????????????????????????????γ?????????????????????????????????????????????????????????????????????????С?????? 1???? ?????γ??????ü???????????????????? 100 ?m ????????? [1]??
?????????????? 4Dcell ????????? С??????????????????????????? 300 um???μ?? 2 ??????????γ???????????????????Щ????????????????С???????????????????????????????????????????????????????????????????????????????????γ??????????????????????????????????????????????????????δ????????? 2???? С???????????????????? 300 ?m ????????? [4Dcell ????]??
REFERENCES [1] Vedula SR, et al. Mechanics of epithelial closure over non-adherent environments. Nat Commun. (2015)
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